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The Populus homeobox gene ARBORKNOX2 regulates cell differentiation during secondary growth.

Identifieur interne : 003466 ( Main/Exploration ); précédent : 003465; suivant : 003467

The Populus homeobox gene ARBORKNOX2 regulates cell differentiation during secondary growth.

Auteurs : Juan Du [États-Unis] ; Shawn D. Mansfield ; Andrew T. Groover

Source :

RBID : pubmed:19737362

Descripteurs français

English descriptors

Abstract

The stem cells of the vascular cambium divide to produce daughter cells, which in turn divide before undergoing differentiation during the radial growth of woody stems. The genetic regulation of these developmental events is poorly understood, however. We report here the cloning and functional characterization of a Populus class-I KNOX homeobox gene, ARBORKNOX2 (ARK2), which we show influences terminal cell differentiation and cell wall properties during secondary growth. In the early stages of secondary growth, ARK2 is expressed broadly in the cambial zone and in terminally differentiating cell types, before becoming progressively restricted to the cambium. ARK2 overexpression and synthetic miRNA-suppression transgenics reveal positive correlations between ARK2 expression level and the timing of cambium formation, the width of the cambial zone and inhibition of cambial daughter cell differentiation. These phenotypes in turn correlate with changes in the expression of genes affecting transcription, cell division, auxin and cell wall synthesis. Notably, wood properties associated with secondary cell wall synthesis are negatively associated with ARK2 expression, including lignin and cellulose content. Together, our results suggest that ARK2 functions primarily to regulate a complex suite of genes that together influence cell differentiation during secondary growth. We propose that ARK2 may represent a co-evolved transcriptional module that influences complex, adaptive wood properties.

DOI: 10.1111/j.1365-313X.2009.04017.x
PubMed: 19737362


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Le document en format XML

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<term>Cellulose (metabolism)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Gene Expression Regulation, Developmental (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Homeobox (MeSH)</term>
<term>Lignin (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Oligonucleotide Array Sequence Analysis (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Plants, Genetically Modified (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (growth & development)</term>
<term>Populus (metabolism)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Wood (growth & development)</term>
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<term>Alignement de séquences (MeSH)</term>
<term>Bois (croissance et développement)</term>
<term>Cellulose (métabolisme)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Différenciation cellulaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Gènes homéotiques (MeSH)</term>
<term>Lignine (métabolisme)</term>
<term>Populus (croissance et développement)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes au cours du développement (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquençage par oligonucléotides en batterie (MeSH)</term>
<term>Végétaux génétiquement modifiés (croissance et développement)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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<term>Plant Proteins</term>
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<term>Populus</term>
<term>Végétaux génétiquement modifiés</term>
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<term>Populus</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en">
<term>Plants, Genetically Modified</term>
<term>Populus</term>
<term>Wood</term>
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<term>Populus</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
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<term>Plants, Genetically Modified</term>
<term>Populus</term>
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<term>Cellulose</term>
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<term>Populus</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
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<term>Données de séquences moléculaires</term>
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<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
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<div type="abstract" xml:lang="en">The stem cells of the vascular cambium divide to produce daughter cells, which in turn divide before undergoing differentiation during the radial growth of woody stems. The genetic regulation of these developmental events is poorly understood, however. We report here the cloning and functional characterization of a Populus class-I KNOX homeobox gene, ARBORKNOX2 (ARK2), which we show influences terminal cell differentiation and cell wall properties during secondary growth. In the early stages of secondary growth, ARK2 is expressed broadly in the cambial zone and in terminally differentiating cell types, before becoming progressively restricted to the cambium. ARK2 overexpression and synthetic miRNA-suppression transgenics reveal positive correlations between ARK2 expression level and the timing of cambium formation, the width of the cambial zone and inhibition of cambial daughter cell differentiation. These phenotypes in turn correlate with changes in the expression of genes affecting transcription, cell division, auxin and cell wall synthesis. Notably, wood properties associated with secondary cell wall synthesis are negatively associated with ARK2 expression, including lignin and cellulose content. Together, our results suggest that ARK2 functions primarily to regulate a complex suite of genes that together influence cell differentiation during secondary growth. We propose that ARK2 may represent a co-evolved transcriptional module that influences complex, adaptive wood properties.</div>
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